Apolipoprotein E genotyping using PCR-GoldMag lateral flow assay and its clinical applications
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Apolipoprotein E genotyping using PCR-GoldMag lateral flow assay and its clinical applications
A polymerase chain reaction-gold magnetic nanoparticles lateral flow assay (PCR-GoldMag LFA) has been developed via integrating multiplex amplification refractory mutation system PCR (multi‑ARMS‑PCR) with GoldMag‑based LFA for the visual detection of single‑nucleotide polymorphisms (SNPs). This assay was applied to genotype Apolipoprotein E (ApoE). ApoE genotyping is important due to the predic...
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Current techniques for single nucleotide polymorphism (SNP) detection require tedious experimental procedures and expensive and sophisticated instruments. In this study, a visual genotyping method has been successfully established via combining ARMS-PCR with gold magnetic nanoparticle (GoldMag)-based lateral flow assay (LFA) and applied to the genotyping of methylenetetrahydrofolate reductase (...
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References 1. Young DS, Bermes EW Jr. Specimen collection and processing-sources of biological variation. In: Burtis CA, Ashwood ER, eds. Tietz textbook of clinical chemistry, 2nd ed. Philadelphia: WB Saunders, 1994:58-101. 2. Lahiri DK, Schnabeh B. DNA isolation by a rapid method from human blood samples: effects of MgC12, EDTA, storage time, and temperature on DNA yield and quality. Biochem G...
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References 1. Matarese V, Stone RL, Waggoner DW, Bernlohr DA. Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 1989;28:245–72. 2. Baier LJ, Sacchettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, et al. An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increase...
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ژورنال
عنوان ژورنال: Molecular Medicine Reports
سال: 2016
ISSN: 1791-2997,1791-3004
DOI: 10.3892/mmr.2016.5768